BD Influx cell sorter

  • BD InfluxTM II cell sorter

This Influx II is equipped with 2 high power lasers: a Coherent Sapphire solid-state 488 nm (200 mW) and a diode-pumped solid-state 457 nm (blue, 300 mW).

Have a look at the fluorescence spectrum at 488 nm and 457 nm.

2 scatter detectors (including a small-particule forward scatter detector) and

7 fluorescence detectors:

  • 530/40 nm (488; FITC/SYBRGreen)
  • 580/30 nm (488; PE/PI)
  • 610/20 nm (488; PE-TR/PI)
  • 692/40 nm (488; PE-Cy5.5)
  • >750 nm (488; PE-Cy7)
  • 480/40 nm (457; CFP)
  • >650 (457; Chlorophyll)

Sorts can be performed using a 70, 86 or 100-micron nozzle tip and collected in a wide range of devices such as microcentrifuge tubes, sort tubes (PP 7 mL), 50 or 15-mL Falcon like tubes, but also microscope slides or well plates. Up to 6 populations of cells can be sorted simultaneously. The sensitivity of this instrument is high and optimal to analyze autofluorescent cells with a low pigment content.

 

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