High resolution microscopy combined with flow cytometry
The ImageStream MKII combines phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. Numerous applications (examples below) for qualitative and quantitative measurements (i.e. cell signaling and intensity, internalization, cell cycle, morphology, cell-cell interaction, colocalization, etc).
Features:
- 12 Channel, 3 laser system
- 20X/40X/60X magnification (motorized & autofocused)
- Extended depth of field
- 5000 cells/sec
- IDEAS software combines image analysis with statistical stringency
- Automated start-up, QC and shutdown procedures
- Analysis workstation use free of charge or download the software for your own computer
Automated analysis with standard applications:
Phagocytosis Nuclear Translocation
Neutrophil phagocytosis of Aspergillus fumigatus NFkB translocation to the nucleus
Customize masks:
Ly6G component mask (A) and erode mask (B) combined to form a membrane mask
Contact Dr. Hou for questions and training. Additional information can be found on the Amnis website.
Publications:
Imaging the dynamic recruitment of monocytes to the blood-brain barrier and specific brain regions during Toxoplasma gondii infection. Schneider CA, Figueroa Velez DX, Azevedo R, Hoover EM, Tran CJ, Lo C, Vadpey O, Gandhi SP, Lodoen MB. Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24796-24807. doi: 10.1073/pnas.1915778116. Epub 2019 Nov 14. PMID: 31727842
CD11a expression distinguishes infiltrating myeloid cells from plaque-associated microglia in Alzheimer’s disease. Shukla AK, McIntyre LL, Marsh SE, Schneider CA, Hoover EM, Walsh CM, Lodoen MB, Blurton-Jones M, Inlay MA. Glia. 2019 May;67(5):844-856. doi: 10.1002/glia.23575. Epub 2018 Dec 26. PMID: 30588668
Neutrophil Caspase-11 Is Required for Cleavage of Caspase-1 and Secretion of IL-1β in Aspergillus fumigatus Infection. Sun Y, Abbondante S, Karmakar M, de Jesus Carrion S, Che C, Hise AG, Pearlman E. J Immunol. 2018 Nov 1;201(9):2767-2775. doi: 10.4049/jimmunol.1701195. Epub 2018 Sep 28. PMID: 30266768
Immunomodulation of the NLRP3 Inflammasome through Structure-Based Activator Design and Functional Regulation via Lysosomal Rupture. Manna S, Howitz WJ, Oldenhuis NJ, Eldredge AC, Shen J, Nihesh FN, Lodoen MB, Guan Z, Esser-Kahn AP. ACS Cent Sci. 2018 Aug 22;4(8):982-995. doi: 10.1021/acscentsci.8b00218. Epub 2018 Jul 2. Erratum in: ACS Cent Sci. 2018 Nov 28;4(11):1593 PMID: 30159395
Evasion of Human Neutrophil-Mediated Host Defense during Toxoplasma gondii Infection. Lima TS, Gov L, Lodoen MB. mBio. 2018 Feb 13;9(1). pii: e02027-17. doi: 10.1128/mBio.02027-17. PMID: 29440572