ImageStream

High resolution microscopy combined with flow cytometry

The ImageStream MKII combines phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. Numerous applications (examples below) for qualitative and quantitative measurements (i.e. cell signaling and intensity, internalization, cell cycle, morphology, cell-cell interaction, colocalization, etc).

Features:

• 12 Channel, 3 laser system
• 20X/40X/60X magnification (motorized & autofocused)
• Extended depth of field
• 5000 cells/sec
• IDEAS software combines image analysis with statistical stringency
• Automated start-up, QC and shutdown procedures
• Analysis workstation use free of charge or download the software for your own computer

Automated analysis with standard applications:

Phagocytosis                                                                                                  Nuclear Translocation

Neutrophil phagocytosis of Aspergillus fumigatus                NFkB translocation to the nucleus

Customize masks:

Ly6G component mask (A) and erode mask (B) combined to form a membrane mask

Contact Dr. Hou for questions and training. Additional information can be found on the Amnis website.

Publications:

Imaging the dynamic recruitment of monocytes to the blood-brain barrier and specific brain regions during Toxoplasma gondii infection.  Schneider CA, Figueroa Velez DX, Azevedo R, Hoover EM, Tran CJ, Lo C, Vadpey O, Gandhi SP, Lodoen MB.  Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24796-24807. doi: 10.1073/pnas.1915778116. Epub 2019 Nov 14.  PMID: 31727842

Neutrophil Caspase-11 Is Required for Cleavage of Caspase-1 and Secretion of IL-1β in Aspergillus fumigatus Infection.  Sun Y, Abbondante S, Karmakar M, de Jesus Carrion S, Che C, Hise AG, Pearlman E.  J Immunol. 2018 Nov 1;201(9):2767-2775. doi: 10.4049/jimmunol.1701195. Epub 2018 Sep 28.  PMID: 30266768