We introduce a method for quantitative hyperspectral optical imaging in the spatial frequency domain (hs-SFDI) to image tissue absorption (μa) and reduced scattering ( μs′) parameters over a broad spectral range. The hs-SFDI utilizes principles of spatial scanning of the spectrally dispersed output of a supercontinuum laser that is sinusoidally projected onto the tissue using a digital micromirror device. A scientific complementary metal–oxide–semiconductor camera is used for capturing images that are demodulated and analyzed using SFDI computational models. The hs-SFDI performance is validated using tissue-simulating phantoms over a range of μa and μs′ values. Quantitative hs-SFDI images are obtained from an ex-vivo sample to spatially resolve concentrations of oxy-, deoxy-, and met-hemoglobin, as well as water and fat fractions. Our results demonstrate that the hs-SFDI can quantitatively image tissue optical properties with 1000 spectral bins in the 580- to 950-nm range over a wide, scalable field of view. With an average accuracy of 6.7% and 12.3% in μaand μs′, respectively, compared to conventional methods, hs-SFDI offers a promising approach for quantitative hyperspectral tissue optical imaging.
Published in the Journal of Biomedical Optics July 2019, by Mohammad Torabzadeh
Hyperspectral imaging in the spatial frequency domain with a supercontinuum source
Authors
Mohammad Torabzadeh,1,2 Patrick A. Stockton,3 Gordon T. Kennedy,1 Rolf B. Saager,4 Anthony J. Durkin,1,2 Randy A. Bartels,3 Bruce J. Tromberg1,2
1Beckman Laser Institute and Medical Clinic (United States)
2Univ. of California, Irvine (United States)
3Colorado State Univ. (United States)
4Linköping Univ. (Sweden)
Citation:
J. of Biomedical Optics, 24(7), 071614 (2019). https://doi.org/10.1117/1.JBO.24.7.071614